ulbp1  (R&D Systems)

Journal: Cellular Oncology (Dordrecht, Netherlands)

Article Title: TAK-981 potentiates doxorubicin immunocide in triple-negative breast cancer by IFN I-dependent NK cell stimulation

doi: 10.1007/s13402-025-01114-0

Figure Lengend Snippet: Modulation of the NKG2D-NKG2D Ligand Axis by a Combination Drug Regimen. ( A ) mRNA expression of MICA in TCGA; ( B ) mRNA expression of MICB in TCGA; ( C ) mRNA expression of ULBP1 in TCGA; ( D ) mRNA expression of ULBP2 in TCGA; ( E ) mRNA expression of ULBP3 in TCGA; ( F - J ) Kaplan-Meier Plotter. NKG2DLs for survival prognosis in Triple-negative breast cancer; ( K ) Western blot to detect the expression of surface receptors NKG2D and NKG2A in NK cell; ( L - M ) RTq-PCR to detect the expression of surface receptors NKG2A and NKG2D in NK cell; ( N ) Representative immunoblots of NKG2DLs in MDA-MB-231 cell; ( O ) Changes of SUMO1 protein in MDA-MB-231 cell detected by immunofluorescence; ( P ) Changes of SUMO1 protein in MDA-MB-468 cell detected by immunofluorescence. Each data point represents the mean ± SD for three biological replicates. ns, no significance, * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: The antibodies employed in this Western blot analysis included GAPDH (dilution 1:1000, #60004-1-Ig, Proteintech, Wuhan, China), SUMO1 (dilution 1:1000, #ab5316, Abcam, Cambridge, England), JAK1 (dilution 1:1000, #3332,Cell Signaling Technology, Boston, USA), p-JAK1 (dilution 1:1000, #74129,Cell Signaling Technology, Boston, USA), STAT1(dilution 1:1000, #82016-1-RR, Proteintech, Wuhan, China), p-STAT1(dilution 1:800, #AF6300, affinity biosciences, Affinity Biosciences, Ohio, USA), NF-κB(dilution 1:1000, #10745-1-AP, Proteintech, Wuhan, China), p- nf-κB(dilution 1:500, #80379-2-RR, Proteintech, Wuhan, China), IFNβ (dilution 1:200, #27506-1-AP, Proteintech, Wuhan, China), ULBP1 (dilution 1:1000, #17715-1-AP, Proteintech, Wuhan, China), ULBP2 (dilution 1:1000, #13133-1-AP, Proteintech, Wuhan, China), ULBP3 (dilution 1:1000, #30058-1-AP, Proteintech, Wuhan, China), NKG2A (dilution 1:1000, #10935-1-AP, Proteintech, Wuhan, China), NKG2D (dilution 1:1000, #ab96606, Abcam, Cambridge, England), MIC A + MIC B (dilution 1:1000, #ab224702, Abcam, Cambridge, England), anti-Goat Anti-Rabbit IgG (H + L) HRP (dilution 1:5000, #S0001, Affinity Biosciences, Ohio, USA), and anti-Goat Anti-Mouse IgG (H + L) HRP (dilution 1:5000, #S0002, Affinity Biosciences, Ohio, USA).

Techniques: Expressing, Western Blot, Immunofluorescence

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Liposome‐Polymer Nanoparticles Loaded with Copper Diethyldithiocarbamate and 6‐Bromo‐Indirubin‐3′‐Oxime Enable the Treatment of Refractive Melanoma

doi: 10.1002/smll.202409012

Figure Lengend Snippet: A) Difference in cytokine levels in isolated serum from B16F10 mice treated with 3 mg/kg of vehicle ( n = 4), LPBIO ( n = 5), LPCuET ( n = 5), and LPBC ( n = 4) at 24h post IV injection showing a notable decrease in inflammatory cytokines and chemokines when treated with LBPC compared to LPCuET, One‐Way ANOVA with Dunnett's correction was used, empty circles represent individual mice and data is shown as mean. B) Heat map showing the difference in the expression of select cytokine and chemokine mRNA extracted from whole ectopic B16F10 tumors of mice treated with the various groups showing a reduction of pro‐inflammatory markers when BIO is used alone or in combination with CuET, data depicted as the geometric mean of the fold change in gene expression (2 ‐ΔΔCt ) from vehicle‐treated mice. C) Representative lung pictures and lesion count in B16F10 lung metastases showing the extent of the tumor burden in mice injected with control B16F10 cells compared to B16F10 injected alongside 3 mg/kg LPBC, or B16F10 cells pre‐treated with 1 and 5 µ m of LPBC for 3h before IV injection, showing that the exposure of cells to LPBC before injection into mice can diminish their ability to form metastases, black nodules represent individual tumors. D,E) Differential fold change in the gene expression profile of surface receptors relevant for NK cell recognition in D) B16F10 cells and E) A375 cells, empty circles represent individual experiments, and data is shown as geometric mean ± SD. F,G) Flow cytometry experiment showing the increase in the proportion of F) NKp44L+ B16F10 cells and G) ULBP1+ A375 cells when treated with LPBC, student's t ‐test with Welch's correction was used, and data is shown as mean. H,I) Flow cytometry experiment showing the proportion and mean fluorescence intensity (MFI) of H) PD1+ and I) CD69+ Jurkat cells when activated in the presence of BIO, CuET, or the combination compared to vehicle control, Welsch's One‐Way ANOVA with Dunnett's 3T correction was used, empty circles represent individual experiments and data is shown as mean.

Article Snippet: For A375 and B16F10 NK receptors, cells were seeded in 6‐well plates and treated with 100 n m of vehicle control or LPBC for 24 h. Single‐cell suspensions were washed with PBS and labeled with primary rabbit anti‐mouse NKp44L (A6142, ABclonal) and primary rabbit anti‐human ULBP1 (A10483, ABclonal) with secondary goat anti‐rabbit (A11008, invitrogen).

Techniques: Isolation, IV Injection, Expressing, Gene Expression, Injection, Control, Flow Cytometry, Fluorescence